Technical background: Orlistat, which can reduce the patient's weight, improve abnormal blood fat and reduce blood pressure., is a kind of safe treatment for obesity drugs . For the first time in 1998 Orlistat marketed in the ?xml:namespace>
The existing production methods: First, Total chemical synthetic method; Second, Microbial ferment to produce Lipp statins, then hydrogenated and synthetized Orlistat.
Because of the complex process of Lipstatin extraction and purification, difficult to obtain high-purityproducts, poorreproducibilitybetween batches, the fermentation unit influence purification process of the fermentation of downstream too much, technicians of Ji'nan Bona biological technology limited company developed chromatography resin SKP-10-4500 for theseparation and purification ofLipstatin after a long periodresearch and development. They improved the original process, the production process is greatly simplified, the labor intensity was greatly reduced, yield is higher, production cost is lower, the production process is safer.
The Introduction of extraction and purification process of Orlistat, Lipstatin, Nebo statins:
1、adding 5% of diatomaceous earth / perlite to fermentation broth。
2、filtering bacteria with plate and frame.
3、extracting fermentation broth with alcohol, concentrating, Microfiltration, extracting with Heptane, removing oil with acetonitrile
4、A column is packed with Polymeric Chromatography Media SKP-10-4500 .Elution is carried out with mobile phase and the eluents are fractionally collected. Lipstatin purity more than 95%.
5、subjected to catalytic hydrogenation
6、A column is packed with Polymeric Chromatography Media SKP-10-4500 .Elution is carried out with mobile phase and the eluents are fractionally collected. Concentrated, and Crystallized The finished product purity more than 99%, the largest single complex 0.10%.The total yield is more than 40%.
If you want to buy our Polymeric Chromatography Media SKP-10-4500 or request sample, please call us. We will be happy to provide you with technical supports.