Epothilone (Epo), which is similar with the biological activity of paclitaxel, is cytotoxic compounds isolated
from the fermentation broth of Sorangium cellulosum. Epothilone which is macrolide compounds with 16
carbon, has variety of biological activities analogues such as Epo A, Epo B and Epo D 。 Epothilone ,
with good water solubility and simple structure and good potential for chemical modification and
paclitaxel-resistant tumor cells with high activity, is an new anticancer drug with the best market potential。
Fermentation unit is low, the highest unit demestic domestic is 100mg / L or so, This puts forward higher
request for technology development of separation and purification . For fermentation extract viscosity,
similar structure of Epo A and Epo B, We develop SKP-10-4100 resin chromatography media,
which makes the separation and purification more simple, the yield can reach more than 70%
using SKP-10-4100 resin chromatography media .
The Separation and purification technology of Epothilone:
First, Fermentation liquid and resin HZ816 filtering together through the sieve filter
Secondly, collecting adsorbent resin, Purified water washing until colorless
Third, washing the resin with 80% ethanol, concentrating under reduced pressure
Fourth, Extracting with ethyl acetate , with decoloring Activated carbon
Fifth, dissolving with concentrated under reduced pressure methanol
Sixth, chromatography withSKP-10-4100resin ,then getting Epothilone B
SKP-10-4100 chromatography resin is a new generation of chromatographic separation
chromatography media which is developed by technicians of Bona Bio-technology Co., Ltd.
Chromatographed pressure is low and speed is flow. Without pressure preparative column,
just ordinary packed chromatography column you can perfectly separate epothilone.
The price is one-tenth of the price of silica filler modified.